METABOLISM OF Cl*-LABELED GLYCEROL AND PYRUVATE BY LIVER IN VITRO* BY CHING-TSENG TENG, MANFRED L. KARNOVSKY, BERNARD

In a previous paper (l), the conversion of labeled pyruvate and of labeled glucose to glycogen, glucose, and COZ by rat liver slices, in vitro, was reported. It is the purpose of the present communication to present experiments on the metabolism of labeled glycerol and of labeled pyruvate by the same experimental system. The results provide quantitative information on the metabolic pathways followed by glycerol and by pyruvate, singly and together, thus permitting a comparison of the effect of one biologically important carbohydrate intermediate upon the metabolism of another. The present experiments include measurements of the incorporation of labeled carbons from glycerol and pyruvate into lipides, proteins, and those phosphorylated intermediates included in the barium-precipitable and alcohol-insoluble fractions, as well as into glycogen, glucose, and COZ.